Hemascein® detects latent bloodstains using novel fluorescein
C20H10Na2O5 as an active component in the formulation.

The aqueous formulation of Hemascein® reveals latent bloodstains based on the peroxidase-reduction of a reduced colorless reagent sprayed over the targeted area. An area suspected of containing latent bloodstains is sprayed with Hemascein® using ABASpray™. The Hemascein® reagent reduced with hydrogen peroxide (colorless) is immediately oxidized to fluorescin by the presence of blood-associated proteins and will fluoresce when excited by a light source between 415 and 480 nm. Once latent bloodstains are located, they can be tested at the crime scene if they are of human origin with the help of Hematrace®, a 10-minute immunochromatographic test. The evidence can then be swabbed and transported for further DNA analysis.
Sensitivity in detecting latent bloodstains
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2 to 5 times when compared to other tools in use |
| DNA recovery of diluted blood |
Yes |
| Recovers finer detail |
Yes |
| Longevity, time for documentation |
Highest when compared to other tools in use |
| Stability |
7 yrs (Room Temperature) |
| Safety |
Fluorescein is routinely used in humans |
| Identify if human origin |
Yes (Hematrace®) |
| Formulation |
Fluorescein containing formulation (aqueous) |
| Requires dark room and eyes to adjust to darkness |
No |
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“HemaScein® addresses the issue of health hazards and liability. A luminol application created substantial liability issue in a claim to purchase a vehicle and the payment of medical bills of an adult and children who were affected by the residue luminol left behind in the application to a vehicle interior. ….investigators reported burning to their hands and face after using luminol……. private citizens filed a claim and received compensation directly due to health issues arising from the use of luminol. … the jurisdiction of record bought motor vehicles that had been processed with luminol.
Hemascein® does not present this liability issue of a residual hazardous material left behind at the scene. The author over the past 11 years in using fluorescein has never received a complaint from a citizen or investigator of direct health issues."– Sergeant Larry Barksdale, Lincoln Police Dept., USA Click here to request a copy of this study
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