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Compare the strengths & weaknesses of various technologies      

Semen Identification Test for the forensic and crime scene use
 
“The ABAcard® test has been extensively validated and shown to be sensitive, specific and rapid” Presidents DNA Initiative National Institute of Justice, U.S. Department of Justice
 
 
Sensitivity
Specificity
Storage
Running
Buffer Required
Extraction
Buffer Required
Extraction Time(incl reagent prep)
Sensitivity Defined in ng/ml for QC purposes
Labor Intensive/
Extensive Prep required
ABAcard® p30
Highest 0.02 µl to 0.0007 µl of semen
Ref
Highest
Ref  
Room Temp, 20 months
No
No
5-30 mins
Yes
(4 ng/ml)
No
Single Step
Semenogelin

Lowest 1 µl of Semen
Ref 1 Ref 2

 Ref5
Refrigeration required(2 buffers both need refrigeration)
Yes & Requires Refrigeration
Yes & Requires Refrigeration
1-2 hours
No
(1 µl of semen)
(Semen varies widely)
Yes
Multiple Step
Cross Over Electrophoresis
Lower 10 µl to 0.35 µl of semen
Highest
Refrigeration required
Yes, Requires refrigeration
Yes, Requires refrigeration
1-3 hours
Yes
(4000 ng/ml)
Yes
Multiple Step, Longest

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Blood Identification Test for the forensic and crime scene use
 
“Currently, the preferred confirmatory test for human blood is Abacus Diagnostics’ ABAcard®Hematrace®” Presidents DNA Initiative National Institute of Justice, U.S. Department of Justice
 
  Sensitivity Specificity Storage Running
Buffer Required		 Extraction
Buffer Required		 Extraction Time Sensitivity Defined in µg/ml for QC purposes so that it can be defended and validated Labor Intensive/ Extensive Prep required
Hematrace® Highest 0.00033 µl of blood
Ref		 Highest
Ref		 Room Temp (20 months) No Yes, Store at Room Temperature 5 minutes Yes
(0.05 µg/ml)		 No
Single Step
Glycophorin Lowest
1 µl of blood		 Lowest
Ref1
Ref2
Ref3		 Refrigeration Required Yes & Requires refrigeration Yes, Requires refrigeration 1-2 hours No
(1 µl of blood)		 Yes
Multiple Preps
Hemastix Higher
0.001 µl of blood		 Lowest Room Temp No No 1 minute No No

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Saliva Identification Test for the forensic and crime scene use
 
SALIgAE® Protocol Presidents DNA Initiative  National Institute of Justice, U.S. Department of Justice
 
  Sensitivity Specificity Buffer Required Extraction Time Ease of Use
Visible result including availability of Spray Mechanism for mapping		 Labor Intensive/Extensive Prep required
SALIgAE ® Highest
Ref		 Higher
Ref		 No 1-5 minutes Yes
Simple Color Change		 No
Ready to use
Salivary Amylase Antibody Lowest Higher Yes, Multiple ones
Needs Refrigeration		 1-2 hours No
Mapping not available		 Yes
Long Prep Time
Radial Gel Diffusion Medium High Yes, Multiple ones
Needs Refrigeration		 30 minutes No
Very cumbersome		 Yes
Very Long
Prep Time
Liquid Phase Amylase Test Highest Lowest Yes, Multiple ones 30 minutes No
Very cumbersome		 Yes
Very Long Prep Time

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