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Compare the strengths & weaknesses of various technologies
     


Semen Identification Test for the forensic and crime scene use
 
“The ABAcard® test has been extensively validated and shown to be sensitive, specific and rapidPresidents DNA Initiative National Institute of Justice, U.S. Department of Justice
 
 
Sensitivity
Specificity
Storage
Running
Buffer Required
Extraction
Buffer Required
Extraction Time(incl reagent prep)
Sensitivity Defined in ng/ml for QC purposes
Labor Intensive/
Extensive Prep required
ABAcard® p30
Highest 0.02 µl to 0.0007 µl of semen
Ref
Highest
Ref
  
Room Temp, 20 months
No
Yes, Store at Room Temperature
5-30 mins
Yes
(4 ng/ml)
No
Single Step
Semenogelin

Lowest 1 µl of Semen

Ref 1 Ref 2
Ref 3 Ref 4Ref 5

Ref5 Ref 6Ref 7 Ref 8

Refrigeration required(2 buffers both need refrigeration)
Yes & Requires Refrigeration
Yes & Requires Refrigeration
1-2 hours
No
(1 µl of semen)
(Semen varies widely)
Yes
Multiple Step
Cross Over Electrophoresis
Lower 10 µl to 0.35 µl of semen
Highest
Refrigeration required
Yes, Requires refrigeration
Yes, Requires refrigeration
1-3 hours
Yes
(4000 ng/ml)
Yes
Multiple Step, Longest

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Blood Identification Test for the forensic and crime scene use
 
“Currently, the preferred confirmatory test for human blood is Abacus Diagnostics’ ABAcard®Hematrace®Presidents DNA Initiative National Institute of Justice, U.S. Department of Justice
 
  Sensitivity Specificity Storage Running
Buffer Required
Extraction
Buffer Required
Extraction Time Sensitivity Defined in µg/ml for QC purposes so that it can be defended and validated Labor Intensive/ Extensive Prep required
Hematrace® Highest 0.00033 µl of blood
Ref
Highest
Ref
Room Temp (20 months) No Yes, Store at Room Temperature 5 minutes Yes
(0.05 µg/ml)
No
Single Step
Glycophorin Lowest
1 µl of blood
Lowest
Ref1
Ref2
Ref3
Refrigeration Required Yes & Requires refrigeration Yes, Requires refrigeration 1-2 hours No
(1 µl of blood)
Yes
Multiple Preps
Hemastix Higher
0.001 µl of blood
Lowest Room Temp No No 1 minute No No

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Saliva Identification Test for the forensic and crime scene use
 
SALIgAE® Protocol Presidents DNA Initiative  National Institute of Justice, U.S. Department of Justice
 
  Sensitivity Specificity Buffer Required Extraction Time Ease of Use
Visible result including availability of Spray Mechanism for mapping
Labor Intensive/Extensive Prep required
SALIgAE ® Highest
Ref
Higher
Ref
No 1-5 minutes Yes
Simple Color Change
No
Ready to use
Salivary Amylase Antibody

Lowest

Ref

Higher

Ref Ref

Yes, Multiple ones
Needs Refrigeration
1-2 hours No
Mapping not available
Yes
Long Prep Time
Radial Gel Diffusion Medium High Yes, Multiple ones
Needs Refrigeration
30 minutes No
Very cumbersome
Yes
Very Long
Prep Time
Liquid Phase Amylase Test

Highest

Ref

Lowest

Ref

Yes, Multiple ones 30 minutes No
Very cumbersome
Yes
Very Long Prep Time

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